Blergs
09-26-2014, 05:17 PM
Posted this at another spot, thought maybe it would help here : )
VVVV
Why peptides you use should be from a recombinant DNA source and not a chemically synthesized source
I want to bring something up about peptides and peptide quality. I see many people going the cheap route but there is a reason that they may be simply wasting their money. That is why I wanted to start a thread about an issue I think is going on related to efficacy of chemically synthesized IGF and most other peptides vs. recombinantly made ones.
There are many threads on here about IGF-1 and other peps giving good gains and I feel some of it may be BS or the person is having a "placebo affect", while others swear peptides are a complete scam and do nothing at all! (not true)... There might be a reason your igf1 cycle didn't give you jack shit?
Why?
I believe most are purchasing IGF-1 (for an example of a popular peptide being used) made by cheap peptide synthesis companies. However, chemical synthesis of IGF-1 lacks the correct disulfide bonds that cause the proper folding and function in the peptide chain. IGF-1 and its many analogs require 3 disulfide bonds to generate the correct folded form of the protein and this can only be accomplished in the body as naturally produced, or in E.coli bacteria as a recombinant source which is similar to how HGH is made (recombinantly).
Insulin is also very similar to the structure of IGF-1 and it also is inactive and/or unstable if synthesized chemically, it has to be made recombinantly or extracted from a biologic source (they used to extract insulin from bovine and HGH from cadavers).
Made with "recombinant DNA" means it is made in bacteria which have data input into it and "fermented" create enzymes that make these intramolecular disulfide bonds so that the protein folds into the right configuration and has the biological function it is supposed to have, having the proper amino chain is NOT enough and it is NOT that simple!
If you look into human clinical trials or current clinical treatment of patients, they now use some of these peptides as part of a therapy protocol and they use recombinant DNA made peptides, not chemically synthesized ones and for good reason.
Here is just one of many studies on how important the correct bonds are, not just the structure>
Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI (http://www.ncbi.nlm.nih.gov/pubmed/8494898) > Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI (http://www.ncbi.nlm.nih.gov/pubmed/8494898)
There are many cheap peptide sellers popping out like CRAZY the last couple years with ridiculously low prices that just doesn't make any sense. So I say if it seems too good to be true, then it probably is!
There is a reason some places are so cheap while others can cost more than 2X or 3X the amount of the cheaper places.
You usually get what you pay for so PLEASE do your research first!
Just because it's pricy doesn't automatically mean its top quality, you still need to research the company.
But if it is really cheap then I would just avoid it because the chances of it being properly made are minimal in this case. IMO
There is a flood of shotty quality peptides coming from china, BE SAFE AND AVOID!
What you want is the more expensive recombinant DNA made peptides! NOT the cheaply made, unstable and largely biologically inactive chemically synthesized peptides!
Many don't even have a clue about all this and that is why I am posting this thread.
Hope it helps someone!
Cheers
VVVV
Why peptides you use should be from a recombinant DNA source and not a chemically synthesized source
I want to bring something up about peptides and peptide quality. I see many people going the cheap route but there is a reason that they may be simply wasting their money. That is why I wanted to start a thread about an issue I think is going on related to efficacy of chemically synthesized IGF and most other peptides vs. recombinantly made ones.
There are many threads on here about IGF-1 and other peps giving good gains and I feel some of it may be BS or the person is having a "placebo affect", while others swear peptides are a complete scam and do nothing at all! (not true)... There might be a reason your igf1 cycle didn't give you jack shit?
Why?
I believe most are purchasing IGF-1 (for an example of a popular peptide being used) made by cheap peptide synthesis companies. However, chemical synthesis of IGF-1 lacks the correct disulfide bonds that cause the proper folding and function in the peptide chain. IGF-1 and its many analogs require 3 disulfide bonds to generate the correct folded form of the protein and this can only be accomplished in the body as naturally produced, or in E.coli bacteria as a recombinant source which is similar to how HGH is made (recombinantly).
Insulin is also very similar to the structure of IGF-1 and it also is inactive and/or unstable if synthesized chemically, it has to be made recombinantly or extracted from a biologic source (they used to extract insulin from bovine and HGH from cadavers).
Made with "recombinant DNA" means it is made in bacteria which have data input into it and "fermented" create enzymes that make these intramolecular disulfide bonds so that the protein folds into the right configuration and has the biological function it is supposed to have, having the proper amino chain is NOT enough and it is NOT that simple!
If you look into human clinical trials or current clinical treatment of patients, they now use some of these peptides as part of a therapy protocol and they use recombinant DNA made peptides, not chemically synthesized ones and for good reason.
Here is just one of many studies on how important the correct bonds are, not just the structure>
Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI (http://www.ncbi.nlm.nih.gov/pubmed/8494898) > Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI (http://www.ncbi.nlm.nih.gov/pubmed/8494898)
There are many cheap peptide sellers popping out like CRAZY the last couple years with ridiculously low prices that just doesn't make any sense. So I say if it seems too good to be true, then it probably is!
There is a reason some places are so cheap while others can cost more than 2X or 3X the amount of the cheaper places.
You usually get what you pay for so PLEASE do your research first!
Just because it's pricy doesn't automatically mean its top quality, you still need to research the company.
But if it is really cheap then I would just avoid it because the chances of it being properly made are minimal in this case. IMO
There is a flood of shotty quality peptides coming from china, BE SAFE AND AVOID!
What you want is the more expensive recombinant DNA made peptides! NOT the cheaply made, unstable and largely biologically inactive chemically synthesized peptides!
Many don't even have a clue about all this and that is why I am posting this thread.
Hope it helps someone!
Cheers