Cloudy masteron and test prop

[dontfearthegear]

Well I shouldn't have tried my new autoclave just yet because apparently i got moisture in my media bottles. I had clear gear before filtering and cloudy after. The masteron i made is cloudy but the prop (was second) you could see the water droplets in the gear. after it filtered. Tiny little bubbles on the bottom.

I should have just sterilized in the oven like i've done millions of times before and not had a problem. Plus it seems pointless that once you grab the media bottles out, air (with bacteria) would quickly enter the open autoclave and hit the bottle tops and septa's. So in reality its not sterile at all.

Question: Ive read that you can bake the oil at 250 for roughly 45 min with a needle in the top of the bottle and that should help evaporate the water out of the gear. My question is should i wait to allow the ba to work (5-7 days) before heating or can i go ahead and heat the bottles asap since it won't matter. ill try and get some pics a little later.

[Enigma]

Since no one has chimed in....
”Sterilizing” gear is done in the filtering process, NOT after packaging. The old school way of oven “sterilizing” went out with the dinosaurs. Dinosaurs as in Jurassic Park III.

Let the oil and water separate and draw from the oil portion only in the vial.

Isn’t there a “How-To” thread somewhere here for the home brewing process?

[Woods]

I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .

I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of course

[dontfearthegear]

Since no one has chimed in....
”Sterilizing” gear is done in the filtering process, NOT after packaging. The old school way of oven “sterilizing” went out with the dinosaurs. Dinosaurs as in Jurassic Park III.

Let the oil and water separate and draw from the oil portion only in the vial.

Isn’t there a “How-To” thread somewhere here for the home brewing process?

I've been brewing for almost 10 years so definitely not new to brewing just new to the autoclave. Maybe my post isn't clear. I brewed my materon and prop and sterilized my media bottles separately. I pulled the media bottles out of the autoclave (sterilized) and they obviously had moisture in them since my brew went cloudy and i saw bubbles of water at the bottom of the media bottles when filtering

[dontfearthegear]

I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .

I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of course

Yeah i know there are other ways but the "proper" way is to use a autoclave if you're asking any professional chemist. So i said f it and bought a autoclave to try to it the right way and now its biting me in the end. I'm gonna bake them at 250 tomorrow with a syringe and see if the moisture will evaporate from the bottles. If not, i'll try your method.

[deebs]

Im old and old school like the dinosaurs. You can definitely bake it that way and be fine.

[bayouboy1]

I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .

I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of courseI cant believe I've never considered using the dishwasher for my bottles that's a great idea bud thanks

I still would soak them in antibacterial soap but I hate washing them

Sent from my SM-J337V using Tapatalk

[BuryMeBig]

Are you using a pressure cooker style clave? Or does yours have a dry cycle? Put your media bottles in your clave with foil around the tops if yours doesn't have a dry cycle, when the cycle is over, put the media bottles in the oven (with foil on the tops) and that will dry them up as you can imagine.

[THX]

Im old and old school like the dinosaurs. You can definitely bake it that way and be fine.

Yes you can, you can also pin gear that haven't been filtered/sterilized with no issues, would you do it though?

"baking" alchohol baths etc, won't make it sterile.

[THX]

Are you using a pressure cooker style clave? Or does yours have a dry cycle? Put your media bottles in your clave with foil around the tops if yours doesn't have a dry cycle, when the cycle is over, put the media bottles in the oven (with foil on the tops) and that will dry them up as you can imagine.

I've been reading a lot about covering erlenmeyers and bottles because of the condensation issue. You can't cover them if you want your equipment to be sterilized. Moisture and pressure has to get in.


dontfearthegear (https://brotherhoodofpain.com/members/23548.html):
There are plenty of things that could have happened. Your filter or the plastic filterfunnel isn't compatible with the solvents you've used. You could have gotten moisture in to your test prop before you even started brewing depending on where you stored it (or how your source stored it).
Moisture could have gotten in while you were heating up and mixing the gear etc.

There are so many factors to take in to consideration.

[THX]

Since a lot of people have asked me about how to sterilize lab equipment, vials etc
I've finally decided to write summary on how to do it.
There are a lot of misconceptions about sterilizing lab equipment. I'm going to try to keep it short and simple
but at the same time give you a little deeper understanding.

You should ALWAYS do your own research and not believe things you read on forums, ALWAYS!

Here is a list of the most important things that you'll need:
Autoclave/pressure cooker
Distilled water
Isopropyl alcohol (isopropanol) or ethanol (both high concentration)
Surgical gloves
Surgical mask

Less important things:
Autoclave pouches/bags
Autoclave tape
Lab wash bottle



First thing you need is the actual device that you're going to sterilize your lab equipment with.

Autoclave = Expensive and can be rather big. You can get a used one cheap, but you can run in to problems with it. This is the most convenient device (except for buying pre-sterilized things)

Steam sterilizer (autoclave) = That's essentially a pressure cooker with a gauge, usually 18 liter. This is your best option. You can get one from ebay fairly cheap.

Pressure cooker = You can find a regular pressure cooker anywhere. Only problem is not all pressure cookers get up to the right pressure/temperature.

The autoclave/pressure cooker has to reach this temperature/pressure:
121°C (250°F) 15psi 15min
or
132°C (270°F) 30psi 3min

It's the steam/pressure/temperature combination that kills off all the "bad things". So you're not allowed to cover your beakers/Erlenmeyer/vials with aluminum foil.

You can also get autoclave bags. Those bags usually have an indicator that changes color if the right temperature is reached. There is also autoclave tape that does the same thing.



Assuming all the lab equipment/vials (which they are if they are new) are washed and cleaned here is what you do:

1.Alcohol (preferably high percentage isopropyl alcohol but ethanol works as well) to clean your working area and lab equipment with.

2.You'll need distilled water (get a big jug) since you can fill "lab wash bottle" and rinse your lab equipment/glassware after cleaning it with alcohol (so no residue is left, that's why high grade alcohol is preferred)
The distilled water is used for the autoclave/pressure cooker as well. It's used because it won't leave any minerals/residue after it evaporates which regular water does.

3.If you decide to get a regular pressure cooker don't forget to buy a "vegetable steamer basket" since you can't place your vials/equipment on the bottom of the pressure cooker.
You don't need that much water in the pressure cooker. 2.5 - 4cm (1 - 1.5 inches) is usually enough. There should be a little space between the water and the bottom of the "vegetable basket" (You'll know how much water you'll need)

4.Follow the instructions of the pressure cooker or steam sterilizer (autoclave) and just set the time yourself (on your phone or something). Usually those steam sterilizers get up to 121°C (250°F)/15psi so 15min should be enough but I'd go with at least 30min.

5. Let the pressure cooker/steam sterilizer cool down. Now you'll notice that all your equipment is wet/steam/drops etc.
Remove/pour out the water and heat it up a little bit again just so your things get dry.



If you guys think that "baking" your things in the oven will sterilize your lab glassware/vials, you're wrong.
Bacterial spores like Bacillus species and Clostridium are usually very heat resistant. It's harder to kill those types of spores with dry heat, the steam, heat and pressure combo neutralizes them.
Here is how:
Have you ever boiled an egg? Well that's pretty much what pressure and heat does to the protein inside of the bacteria, virus etc.

For those guys that think that alcohol will sterilize stuff, wrong again. Alcohol is a DISINFECTANT, spores are resistant to this method.


With all this said, you shouldn't be paranoid. Some UG-labs don't even bother sterilizing
their vials, some don't even filter their oils. Heard stories about people pinning unfiltered
gear with no issues at all.
But what if your body can't handle that bacteria/virus etc. Then you're fucked. Best case scenario
you just get the flu, worst case, amputation and/or death.
Hope this kills some of the misinformation people are spreading.

At the end of the day the only thing that we can do is minimize the risk of contaminating our gear.
Stay safe bros! //THX