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    Thread: Washing vials and rubber stoppers - Enigma, thoughts?

    1. #11
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      Filters for my 6 foot unit cost $1100usd so you got a deal!

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      Washing vials and rubber stoppers - Enigma, thoughts? Washing vials and rubber stoppers - Enigma, thoughts?
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    4. #12
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      Quote Originally Posted by Enigma View Post
      Filters for my 6 foot unit cost $1100usd so you got a deal!
      I thought so as well! I hope you are doing well my friend!

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      I used to have a big ass autoclave. I sold it because a local hospital paid me well for it and it was a PITA waiting for it to finish cycling. I started buying depyrogenated reams of vials. Then I switched to RFS vials and started using the oven bag/oven method. My first order of vials came in doubled autoclave bags. The exact same packs as the depyro’d. My old autoclave would have held 8 of the reams probably.
      It was also pretty ridiculous to have that big autoclave in a home lab. Now that we are in a building with medical offices, there’s room for it AND I could have provided autoclave services for other tenants. Shit!
      Live and learn. It’s been an enlightening process.

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      Quote Originally Posted by Enigma View Post
      Filters for my 6 foot unit cost $1100usd so you got a deal!
      Quote Originally Posted by Enigma View Post
      I used to have a big ass autoclave. I sold it because a local hospital paid me well for it and it was a PITA waiting for it to finish cycling. I started buying depyrogenated reams of vials. Then I switched to RFS vials and started using the oven bag/oven method. My first order of vials came in doubled autoclave bags. The exact same packs as the depyro’d. My old autoclave would have held 8 of the reams probably.
      It was also pretty ridiculous to have that big autoclave in a home lab. Now that we are in a building with medical offices, there’s room for it AND I could have provided autoclave services for other tenants. Shit!
      Live and learn. It’s been an enlightening process.
      Autoclaving services....hmmm.... that’s an idea. I know folks are always buying “pre-sterilized vials”, however, I wonder if the vials are truly sterilized in some instances. I’m sure there are cases in which sellers of “pre-sterilized” vials just cap vials and call it “sterilized”. The average joe will probably not have any of the vials tested (if there is such a process).

      just thinking out loud.

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    10. #15
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      Quote Originally Posted by JTH View Post
      Autoclaving services....hmmm.... that’s an idea. I know folks are always buying “pre-sterilized vials”, however, I wonder if the vials are truly sterilized in some instances. I’m sure there are cases in which sellers of “pre-sterilized” vials just cap vials and call it “sterilized”. The average joe will probably not have any of the vials tested (if there is such a process).

      just thinking out loud.
      Only way to know is if you sterilize them yourself.
      As I've told you before, some UG-labs in some countries don't sterilize vials at all. I know this for a fact. I don't know much about how UG-labs run their businesses nowadays though.
      When you get vials they are clean and packed so nothing will get in to them. They aren't sterile though.
      When the vials are sterilized and sealed they aren't sterile forever. There are some rules/regulations of how long after they been sterilized they are considered sterile (talking about empty vials now). These regulations are probably just to stay on the safe side.

      I've also mentioned this before but I'm doing it again. Rinsing your equipment, vials, rubber stoppers with alcohol only disinfectants them, alcohol doesn't sterilize them. This means that you have to sterilize you rubber-stoppers as well, not just give them an alcohol bath.

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    12. #16
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      THX, your last paragraph is exactly why I typically type in “steriize”. Disinfect, sterilize, depyrogenated, aseptic...lots of terms that sort of mean the same thing. Sort of.
      We are just trying to minimize the risk of infection. So many variables. Once you pierce the stopper, you’ve likely inoculated the vial with something. Wiping the vial top, the injection site etc. are other things we do. Bathing stoppers in alcohol may very well disinfect them but I agree, won’t “sterilize” them. It is just another step to take tok reduce the risk of infection. This is why we add the BA into our products to make them “bacteriostatic”.
      Two things most people don’t realize:
      The actual evaporation of isopropanol is what “kills” pathogens via “drying” them out. (over simplified)
      Wiping your injection site doesn’t prevent infection, it only minimizes the risk. Many things such as Staphylococcus can’t be killed by doing these wiping. It is EVERYWHERE on our bodies and extremely virulent/persistent.
      All we can do is take steps that are practical for our purposes. Making your own drugs may be risky, but trusting other to make them for you is more of a risk, imo. It’s the very reason I got started doing this. I am sure plenty of these labs like our sponsors here do the best they can to make safe products. Making customers sick is not such a good long term business model.

      Answering JTH’s last post, I too have the suspicion that these “sterile, empty vials” and the ones you get from conversion kits (do they still sell these?) are far from “sterile”. Even the guys who sell them I suspect buy depyrogenated vials but make a saleable package in the open air.
      The reality is that most people don’t have laminar flow benches or BSC. It isn’t even the cost that is prohibitive, it’s having to explain what that giant contraption is in your house when you have visitors. To me, that’s a much larger problem than cost and procurement.

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    15. #17
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      And, THX, every time I see your posts I chuckle because I worked in the building where they filmed THX1138 (and Gattaca) for many years. Also know quite a few people who work(ed) at Lucasfilms.

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      Quote Originally Posted by Enigma View Post
      And, THX, every time I see your posts I chuckle because I worked in the building where they filmed THX1138 (and Gattaca) for many years. Also know quite a few people who work(ed) at Lucasfilms.
      some have asked me what it means/where it's from, it's just a random thing I used as a nickname and kept it.

      Wish I had a proper lab, nowadays a can't even find a reliable powder source...

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    19. Washing vials and rubber stoppers - Enigma, thoughts?
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    Washing vials and rubber stoppers - Enigma, thoughts? Washing vials and rubber stoppers - Enigma, thoughts?