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Cloudy masteron and test prop
Well I shouldn't have tried my new autoclave just yet because apparently i got moisture in my media bottles. I had clear gear before filtering and cloudy after. The masteron i made is cloudy but the prop (was second) you could see the water droplets in the gear. after it filtered. Tiny little bubbles on the bottom.
I should have just sterilized in the oven like i've done millions of times before and not had a problem. Plus it seems pointless that once you grab the media bottles out, air (with bacteria) would quickly enter the open autoclave and hit the bottle tops and septa's. So in reality its not sterile at all.
Question: Ive read that you can bake the oil at 250 for roughly 45 min with a needle in the top of the bottle and that should help evaporate the water out of the gear. My question is should i wait to allow the ba to work (5-7 days) before heating or can i go ahead and heat the bottles asap since it won't matter. ill try and get some pics a little later.
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Since no one has chimed in....
”Sterilizing” gear is done in the filtering process, NOT after packaging. The old school way of oven “sterilizing” went out with the dinosaurs. Dinosaurs as in Jurassic Park III.
Let the oil and water separate and draw from the oil portion only in the vial.
Isn’t there a “How-To” thread somewhere here for the home brewing process?
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I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .
I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of course
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Originally Posted by
Enigma
Since no one has chimed in....
”Sterilizing” gear is done in the filtering process, NOT after packaging. The old school way of oven “sterilizing” went out with the dinosaurs. Dinosaurs as in Jurassic Park III.
Let the oil and water separate and draw from the oil portion only in the vial.
Isn’t there a “How-To” thread somewhere here for the home brewing process?
I've been brewing for almost 10 years so definitely not new to brewing just new to the autoclave. Maybe my post isn't clear. I brewed my materon and prop and sterilized my media bottles separately. I pulled the media bottles out of the autoclave (sterilized) and they obviously had moisture in them since my brew went cloudy and i saw bubbles of water at the bottom of the media bottles when filtering
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Originally Posted by
Woods
I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .
I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of course
Yeah i know there are other ways but the "proper" way is to use a autoclave if you're asking any professional chemist. So i said f it and bought a autoclave to try to it the right way and now its biting me in the end. I'm gonna bake them at 250 tomorrow with a syringe and see if the moisture will evaporate from the bottles. If not, i'll try your method.
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Im old and old school like the dinosaurs. You can definitely bake it that way and be fine.
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Post Thanks / Like - 0 Thanks, 1 Likes, 0 Dislikes
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Originally Posted by
Woods
I'd say take it out of the bottles and reheat it bro add bout 3% BB then refilter it . That will clear it up .
I put my bottles in the disherwasher then alcohol bath let air dry then fill them . Never had a problem doing it that way . This is all in my opinion of course
I cant believe I've never considered using the dishwasher for my bottles that's a great idea bud thanks
I still would soak them in antibacterial soap but I hate washing them
Sent from my SM-J337V using Tapatalk
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Are you using a pressure cooker style clave? Or does yours have a dry cycle? Put your media bottles in your clave with foil around the tops if yours doesn't have a dry cycle, when the cycle is over, put the media bottles in the oven (with foil on the tops) and that will dry them up as you can imagine.
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Originally Posted by
deebs
Im old and old school like the dinosaurs. You can definitely bake it that way and be fine.
Yes you can, you can also pin gear that haven't been filtered/sterilized with no issues, would you do it though?
"baking" alchohol baths etc, won't make it sterile.
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Originally Posted by
BuryMeBig
Are you using a pressure cooker style clave? Or does yours have a dry cycle? Put your media bottles in your clave with foil around the tops if yours doesn't have a dry cycle, when the cycle is over, put the media bottles in the oven (with foil on the tops) and that will dry them up as you can imagine.
I've been reading a lot about covering erlenmeyers and bottles because of the condensation issue. You can't cover them if you want your equipment to be sterilized. Moisture and pressure has to get in.
dontfearthegear:
There are plenty of things that could have happened. Your filter or the plastic filterfunnel isn't compatible with the solvents you've used. You could have gotten moisture in to your test prop before you even started brewing depending on where you stored it (or how your source stored it).
Moisture could have gotten in while you were heating up and mixing the gear etc.
There are so many factors to take in to consideration.
Last edited by THX; 04-22-2019 at 08:04 AM.
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