Thread: Transferring oil from media bottle to vials

how are you able to sterilze a septa cap without melting it ? alcohol bath?

Originally Posted by SloppyJ

The easiest solution is a septa cap. That's the best way to do it. If you leave it open then you're risking contamination. I've found I can get about ~500ml out of a septa before it starts to leak from the puncture holes and be sure never to puncture in the same spot. Use the septa with a 60ml syringe and a 18g or 20g draw needle.

how do you sterilize the septa cap without melting it?

Originally Posted by SloppyJ

I’ve put them in the pressure cooker before but that seems to weaken them and they are more prone to leaking. You can also do dry heat in your oven but I imagine you’ll have the same issues.

I’ve just been dunking them in alcohol and holding them in my hand (with gloves) to let them air dry. It’s been working great.

Don’t forget to use the UV lamp.


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Originally Posted by SloppyJ

That’s only for the high rollers!!

so you’re poor now, is that it? For anyone reading this, and don’t know about the UV lamp, get one and don’t ever worry about sterilization again in your lab. Ole Sloppy ain’t poor he’s filthy rich he just doesn’t like to show off


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Can you give me an idea of a specific uv lamp to Buy bro? Is it a small 1 or large scale?

Originally Posted by wls4runner2110

Caustic, trying to shoot you a PM but it says your inbox is full.

You can try it again, I tried to clean it out


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